īiasini M, Bienert S, Waterhouse A, ArnoldK SG, Schmidt T, Kiefer F, Cassarino TG, Bertoni M, Bordoli L, Schwede T (2014) SWISS-MODEL: modeling protein tertiary and quaternary structure using evolutionary information. (00)80142-Xīates LS, Waldren RP, Teare ID (1973) Rapid determination of proline for water stress studies. īajji M, Lutts S, Kinet JM (2000) Physiological changes after exposure to and recovery from polyethylene glycol- induced water deficit in roots and leaves of durum wheat (Triticum durum Desf.) cultivars differing in drought resistance. Īrnon DI (1949) Copper enzymes in isolated chloroplasts polyphenoloxidase in beta vulgaris. This will help to increase our understanding of drought tolerance/ sensitivity in cotton plants at the molecular level.Īhmad A, Javed MR, Rao AQ, Khan MA, Ahad A, Sala UD, Shahid AA, Husnain T (2015) In-silico determination of insecticidal potential of Vip3Aa-Cry1Ac fusion protein against Lepidopteron targets using molecular docking.
Hence, our results pave the way to utilize the mutagenized known genes for increasing endurance of plants under drought stress. Transgene inheritance into the T1 progeny plants was confirmed by amplification through PCR and integration through Southern blot. Furthermore, the T1 transgenic seedlings containing pmGP1 mutated construct showed longer roots under desiccation stress imposed by 5% PEG. Moreover, relative membrane permeability and the transpiration rate were reduced significantly at P ≤ 0.0001 and P ≤ 0.001 respectively in transgenic plants under drought stress. It was revealed that leaf relative water content, total chlorophyll content, CO 2 assimilation as net photosynthesis, stomatal conductance, total soluble sugars and proline content was significantly increased at P ≤ 0.0001 and P ≤ 0.001 in both the pmGP1 and pGP1 transgenic plants as compared to non transgenic control plants. PCR and semi-quantitative PCR analyses confirmed the amplification and expression of transgene in transgenic plants. Transgenic cotton plants were developed through Agrobacterium mediated genetic transformation by using mutated pmGP1 and non mutated pGP1 constructs under CaMV35S promoter. GUSP1 ( Gossypium arboreum USP) is one of such proteins its amino acids were mutated after in silico simulations including homology modeling and molecular docking analysis. Universal stress proteins belong to Pfam00582 which enables several plants to cope with multiple stresses via ATP binding. Cotton crop suffers shortage of irrigation water at reproductive stage which reduces the yield and fibre quality.
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